Making the diagnosis of Cystic Fibrosis
The Leeds Method of Management. April, 2008. Making the diagnosis of Cystic Fibrosis [online]. Leeds Regional Adult and Paediatric Cystic Fibrosis Units, St James's University Hospital, Leeds, UK. Available from http://www.cysticfibrosismedicine.com
In most cases the diagnosis of cystic fibrosis (CF) is clear but paradoxically with increased understanding of the biochemistry of the disease it may be difficult, in a minority of cases, to say definitely if someone has CF or not. We now know that the amount of functional CFTR (chloride channel) on the cell surface is very variable and can range from virtually nothing (affected by CF) to normal (not a carrier) and to almost any level in between. The level in any individual depends on which and how many CF causing mutations are present and possibly on other factors not yet understood. In routine clinical practice it is not possible to directly quantify the amount and efficiency of the CFTR in respiratory epithelial cells. Investigations such as sweat tests, and electrophysiological measurements on nasal and bowel mucosa, semi quantify the efficiency of the CFTR in cells of the sweat gland, nose and bowel respectively. It is difficult to know where to draw a cut off above which someone is labeled as having CF and below which they are reassured that they do not have CF. On occasions different tests give conflicting results and even the same test when repeated may give variable results. Any such cut off is likely to be incorrect in a number of cases. The clinical outcome of CF can vary significantly even for the same genotype (genetic mutation). It is clear that outcome depends on a complex interaction between CF genotype, other putative modifier genes and the environment. Therefore CF should remain a clinical diagnosis (Rosenstein, 2002).
To allow for this heterogeneity (variability) and to avoid inappropriately labelling someone with a life shortening disease it has been suggested that there should be three diagnostic categories; CF unlikely, non classic CF, and classic CF. This acknowledges that it is very difficult to exclude CF but recognises that there are a group of patients who may need long term follow up and possible intervention depending on progression (De Boeck et al, 2006).
The vast majority of individuals with CF present as clear cut cases. Generally such patients have at least one undisputedly abnormal sweat test, two identified CF causing mutations and a phenotype (clinical presentation) compatible with CF (chronic sinopulmonary disease, nutritional abnormalities, salt loss syndromes or male infertility because of obstructive azoospermia).
Cystic fibrosis (CF) may present in several different ways and varies with patient age. In this section we provide a brief summary of some of the important clinical features which result in a diagnosis.
Presentation following screening
Over the next three years neonatal screening for CF will be introduced to all of the UK (http://www.ich.ucl.ac.uk/neonatal). In the future most patients will present as a result of a positive neonatal screening test. However, it is very important to continue to suspect CF and perform appropriate investigations if a child or adult presents with suggestive symptoms. It is estimated that neonatal screening will miss up to 5% of children born with CF. In addition, older children and adults, who have not had neonatal screening, will continue to present with symptoms for some time.
Presentation following meconium ileus
In 15% to 20% of newborn
infants with CF the bowel is blocked by sticky secretions. The baby has
signs of intestinal obstruction soon after birth with bilious vomiting,
abdominal distension and delay in passing meconium. The obstruction can
often be relieved by Gastrografin® enemas but some infants require
an operation. The outlook for these infants is now good as a result of
the impressive improvements in neonatal surgery, anaesthesia and nutritional
Over 90% of individuals with CF have intestinal malabsorption (Morgan et al, 1999). This is usually evident in infancy but may be less apparent in breast fed newborns. The main cause of malabsorption is a severe deficiency of pancreatic enzymes and bicarbonate. There is also evidence that the transport of some other substances across the wall of the intestine is abnormal. Fortunately, treatment with acid-resistant pancreatic extracts will control malabsorption in most patients (Littlewood & Wolfe, 2000; Littlewood et al, 2006).
Presentation with repeated respiratory infections
Most untreated patients with CF have recurrent chest infections, usually from an early age (Armstrong et al, 2005). The viscid (sticky) mucus in the airways predisposes the lungs to bacterial infections that, once established, are difficult to eradicate. The extent and severity of the damage to the bronchial tubes and lungs from these infections is the main factor in determining the physical state and survival of the patient. However, with early intensive antibiotic treatment, particularly by intravenous and nebulised routes, most people with CF will remain free of significant chest problems throughout childhood. Some patients with respiratory symptoms and bronchiectasis attending general respiratory clinics remain undiagnosed and formal screening with sweat tests and genetics should be undertaken in any patient where CF is a differential diagnosis.
Other clinical presentations
Cystic Fibrosis may present in other ways including neonatal or chronic liver disease, nasal polyposis, asthma, rectal prolapse, bowel obstruction, chronic sinusitis, heat exhaustion from salt depletion, male infertility, growth failure or pancreatitis (Masaryk & Achkar, 1983; Conway et al, 2002).
Presentation of CF in adult life
Cystic Fibrosis is diagnosed in adults with increasing frequency. In Toronto, before and after 1990, 3% and 18% of new diagnoses respectively have been in adults (Gilljam et al, 2004). The spectrum of presentation is different to that seen in children; pancreatitis 4%, genetic screening 9%, pulmonary and gastrointestinal symptoms 22%, infertility 26% and pulmonary symptoms 39% (Gilljam et al, 2004). It is important to recognise that a late diagnosis in an adult patient does not always equate with mild disease (Peckham et al, 2006).
These criteria assist in the diagnosis of “classic CF” and are applicable to most straightforward cases
|Table 1. The USA Cystic Fibrosis Foundation consensus panel criteria for establishing the diagnosis of CF (Rosenstein & Cutting, 1998).|
There are a minority of patients who may present in a variety of ways, in whom it proves difficult to confirm, or importantly, to exclude CF. Patients may present with a borderline sweat chloride between 30-60 mmol/l and any combination of the following:
1) Elevated initial
IRT on screening
“Non classic” CF describes patients with a CF phenotype in at least one organ system (table 2) and borderline sweat test results with insufficient evidence from genotype or electrophysiology to support the diagnosis. These patients may have a very different prognosis to patients with “classical CF” but some may develop progressive lung disease as a result of chronic airway infection in adult life (Conway et al, 2002; Peckham et al, 2006; O’Sullivan et al, 2006). Therefore, these patients should have follow-up. Preventative and acute treatment may be necessary depending upon individual circumstances and evidence of the development of lung disease. Care may involve full CF follow-up or only annual review.
|Table 2. WHO List of single organ disease phenotypes associated with CFTR mutations (Joint Working Group of WHO/ICF (M)A/ECFS/ECFTN, 2001)|
|The sweat test
The sweat test remains central to the diagnosis of CF and it is essential that it is performed by experienced personnel in accordance with national guidelines (National Committee for Clinical Laboratory Standards, 2000; Association for Clinical Biochemistry, 2002). There is controversy as to what should be considered a borderline sweat chloride. Original guidelines suggested a sweat chloride of 40–60 mmol/l, 40 mmol/l representing the mean +2 standard deviations in carriers. Recent evidence suggests that a proportion of patients with “classical CF” have chloride concentrations of 30-60 mmol/l. Sweat chloride concentrations of 30-60 mmol/l are seen in about 4% of sweat tests and 23% of these will subsequently be found to have two CF causing mutations. CF affected patients occur with equal frequency in the 30-40 mmol/l as they do in the 40-60 mmol/l ranges (Lebecque et al, 2002). The sweat test remains the gold standard for confirming the diagnosis of CF and must conform to agreed standards (Littlewood, 1986; Green et al, 2007).
|Table 3. Required standards for sweat tests|
When the delta F508 gene was identified in 1989, clinicians hoped that genetic testing would provide a sensitive and specific diagnostic test. Unfortunately this has not proved to be the case. If a patient has two known CF mutations the diagnosis is confirmed. There are, however, more than 1500 different CFTR mutations and in routine practice it is only possible to check for approximately 100 of these (for an up-to-date register consult the World Wide Web site at http://www.genet.sickkids.on.ca/cftrl). It is therefore possible to confirm the diagnosis of CF by means of the genotype but not possible to exclude it. There are some mutations that may not be enough alone to confirm the diagnosis. R117H may fall into this category and further genetic tests may be required (Chmiel et al, 1999) although there is some debate about this. Most DNA diagnostic laboratories will screen for the most common mutations, although there will be significant variation according to the population ethnicity. The frequency of different mutations in the Yorkshire region is shown in table 4.
|Table 4. Incidence of cystic fibrosis mutations in the Yorkshire population (UK)|
These tests assess a voltage across the membrane in the nose that correlates with the transport of sodium across cell membranes. The characteristic ion transport observed in the respiratory epithelium of patients with CF differs from the pattern of NPD found in individuals with normal healthy epithelia (Knowles et al, 1995; Delmarco et al, 1997; Wilson et al, 1998). To assist in the evaluation of difficult cases a complete bioelectrical profile should be carried out. This includes basal potential difference, response to perfusion with amiloride and to a chloride free solution in conjunction with isoproterenol and ATP. Similar electrophysiological tests can be performed across small bowel mucosa biopsies (Veeze et al, 1991).
With the widespread introduction of neonatal screening, the majority of cases of CF will present with a positive neonatal screening test and will be found to have two disease causing mutations. We advise that all patients should continue to have a confirmatory sweat test. In those patients in whom it proves difficult to confirm or exclude the diagnosis a category such as “non classical CF” will prove useful. Each of these patients should be considered individually and evidence for respiratory disease and other chronic problems should be sought. Depending on symptoms, examination findings and investigations these patients may start on a CF like management regimen which can be reduced, withdrawn or intensified as determined by the individual’s progress. Various classifications and diagnostic algorithms exist and can be helpful in difficult cases (Bush & Wallis, 2000; De Boeck et al, 2006).
It is difficult to say with absolute confidence that someone with a phenotype compatible with CF does not have CF, so a diagnostic label such as “CF unlikely” is useful. In such patients alternative diagnoses such as Primary Ciliary Dyskinesia, Shwachman-Diamond syndrome, gastro-oesophageal reflux and immunodeficiency should be pursued.
Sweat test and genetics as described above.
Microbiology sputum/cough swab culture/bronchoscopy.
Chest x-ray, CT scan for bronchiectasis, Sinus CT scan.
fat soluble vitamin levels, faecal elastase (Cade et al, 2000),
faecal fat assessment (Walters et al, 1990), quantitative faecal
fat estimation (if available)
Clinical examination of male genitalia, semen analysis (when appropriate), ultrasound examination of testis.
Exclusion of other
diagnoses such as ciliary structure and function, immunological deficiency,
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